Rapid monitoring of reconstituted Wilms' tumor suppressor gene-specific t lymphocytes after allogeneic stem cell transplantation using quantitative real time Polymerase chain reaction
نویسندگان
چکیده
Results Both WT1 antigens induced significant quantities of IFN-g mRNA production after 3 h in the qRT-PCR protocol. Among 18 samples of HLA-A2+, specific responses were detected in 27.8% (5/18) when Db126 peptide, compared to 16.7% (3/18) in the case of WH187 peptide. On the other hand, no detection (0/18) was obtained with ELISPOT, when both WT1 peptides were tested. In four HLA-A2samples neither qRT-PCR nor ELISPOT detected any reconstituted WT1-reactive T cells in response to both peptides. As a result, the measurement of IFN-g mRNA by qRT-PCR can be used to detect CTL responses 3 h after WT1 peptides stimulation of PBMCs. Discussion In conclusion, qRT-PCR allows rapid monitoring of WT1-reactive CTLs reconstitution after allogeneic SCT using patient’s PBMCs.
منابع مشابه
Combined usage of Wilms' tumor gene quantitative analysis and multiparameter flow cytometry for minimal residual disease monitoring of acute myeloid leukemia patients after allogeneic hematopoietic stem cells transplantation
High expression of the Wilms' tumor gene (WT1) in acute myeloid leukemia (AML) has been considered as a sensitive marker of minimal residual disease (MRD). The present study investigated the significance of quantitative analysis of WT1 mRNA, combined with multiparameter flow cytometry (MFC) regarding its efficacy and prognostic as well as relapse prediction value for leukemia patients with hema...
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